Analytical, bioactivity and stability studies on strobilanthes crispus L,. bremek and sonchus arvensis L. extracts
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Date
2008
Authors
Afrizal
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Abstract
The purpose of this study was to standardize the leaf extracts (Ew, E7ac, Em,
Eac and Em-sox) of Strobilanthes crispus L. Bremek and Sonchus arvensis L.
for preclinical studies. The standardization work was divided into three steps:
chemical profiling (analytical and phytochemical studies), biochemical profiling
(crystal growth inhibition, antioxidant and stability studies) and biological
profiling (antiangiogenic studies).
Secondary metabolites detected in S. crispus leaves were a-sitosterol,
campesterol, phytol and stigmasterol, whereas lupeol, phytol and a-sitosterol
were detected in S. arvensis leaves. Tritriacontane and stigmasterol were
isolated from S. crispus leaves whilst lupeol and quercetin were isolated from S.
arvensis leaves.
The inhibition indices of Ew, E7ac, Em, Eac, and Em-sox from S. crispus to
inhibit the growth of calcium oxalate crystals were 0.2233 ± 0.0875, 0.1861 ±
0.0124, 0.1587 ± 0.0264, 0.1830 ± 0.0335, and 0.2081 ± 0.0166, respectively.
The values for similar extracts for S. arvensis were 0.3375 ± 0.0157, 0.1994 ±
0.0257, 0.1938 ± 0.0662, 0.1347 ± 0.0439, and 0.3157 ± 0.0457, respectively.
The highest antioxidant activity on FRSA to DPPH, xanthine oxidase activity
and prevention the bleaching of J3-carotene by linoleic acid of S. crispus extracts
are E7ac, Em and Em-sox, respectively whilst those of S. arvensis extracts are
E7ac, Eac and Em, respectively. The percentages of phenolic content from
these plants extract were also determined. Coefficient value (R2
) between their
phenolic contenfand FRSA were 0.93 (S. arvensis) and 0.40 (S crispus). In the
prevention of bleaching of ~-carotene by linoleic acid, effect of protein was more
than that of polysaccharide.
In accelerated stability studies, the extracts (Ew, Em, and Eac) stored at room
temperature (25 °C, 60% RH) was highest when compared to stored at 40 °C
(75%RH), 50 °C (85% RH) and 60 °C (85% RH). Shelf life (too) of Eac, Em and
Ew from S. crispus stored at room temperature was 2.14, 2.17 and 1.94
months, respectively. Meanwhile the t00 of similar extracts from S. arvensis was
2.10, 7.89 and 3.50 months, respectively. The too of stigmasterol in Em and Eac
from S. crispus stored at room temperature was 3.60 and 2.63 months
respectively, whilst lupeol in Em and Eac from S. arvensis was 2.51 and 2.22
months respectively.
In preliminary antiangiogenic studies, the results showed that inhibition
percentages of Ew and Em from S. crispus are 16.67 and 6.25% respectively,
whilst those of Ew and Em from S. arvensis are 11.06 and 8.65% respectively,
exhibiting that these plants possess the potential to prevent or cure
angiogenesis related diseases.
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Keywords
Stability studies , Strobilanthes crispus L. bremek , Sonchus arvensis L. extracts