Uv-Vis Spectrophotometric-Based Metabolite Profiling Of Clinacanthus Nutans Leaves Possessing Antioxidant Activity
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Date
2015-08
Authors
Rezaei, Khansa
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Publisher
Universiti Sains Malaysia
Abstract
A metabolomics approach is an unbiased qualitatively and quantitatively comprehensive study of all the existing metabolites in a biological sample. Plant-based metabolomics seek to identify plant metabolites based on a wide-scale phytochemical analysis. Biomedical composition of plant and its usage in nutrition and medicine have drawn universal attention to the plant metabolomics. This study aims to detect and identify potential active metabolites responsible for antioxidant activity in Clinacanthus nutans (Burm.f) Lindau (C. nutans) leaves extracts using UV-Vis spectrophotometric-based metabolomics approach. Ultrasonication method was applied for the leaf extraction of this prominent medicinal plant using 4 different polarities of solvents. Spectrophotometric data were transformed to SIMCA software version 13.0.3 (Umetrics AB, UmeƄ, Sweden) for multivariate data analysis (MVDA) using principal component analysis (PCA), partial least squares to latent structures (PLS), and orthogonal PLS discriminant Analysis (OPLS-DA). This chemometric analysis was applied to differentiate between various extracts of C. nutans. All models had high reproducibility and predictive ability based on the various diagnostics tools. OPLS-DA showed the clearest discrimination of the 4 clusters of the extracts (p-value of less than 0.0001) i.e. petroleum ether, chloroform, ethanol, and aqueous. The discrimination of the 4 extracts were recorded with wavelengths of 266, 267, 265 nm in PLS and 332, 333, 331 nm in OPLS-DA. Ethanol and water extracts have the positive correlation with antioxidant activity (E > W). Moreover, ethanolic extract of C. nutans leaves showed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity compared to other extracts. Potential active compounds responsible for antioxidant activity in this medicinal plant ethanolic extract were metabolites with the wavelength ranged from 303 to 365 nm such as orientin, homoorientin, shaftoside, vitexin, isovitexin, and caffeic acid. This study highlighted the potential of using UV-VIS spectrophotometry for metabolomic approach to assess metabolite variation in samples and moving towards a comprehensive knowledge of plants with antioxidant activity.
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Keywords
Metabolomics