A study of human choline kinase a (hCK-a) in patients with ischemic heart disease (ihd) and heal thy volunteers
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Date
2008
Authors
Chia, Phee Phee
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia.
Abstract
Ischemic heart disease (IHD) is predicted to become the dominant cause of mortality worldwide by 2030. As a silent yet eventually fatal disease, prevention and early detection through discovery of cardiac biomarker are the best resolution for IHD. Human choline kinase a (hCK-a) was investigated for its possibility as a cardiac biomarker in this study. The aim of this study is to investigate the differences in the protein level and mRNA expression of hCK-a between IHD patients and healthy controls. Blood samples from 10 IHD patients and 10 healthy volunteers were analyzed for mRNA expression using RT-PCR. Western blot was performed as a checkpoint for detection of hCK-a protein in blood. Subsequently, ELISA test was conducted to measure the protein concentration of hCK-a protein. C-reactive protein (CRP) was used as a standard reference in this study to provide a parameter to distinguish between both study groups. Statistical analysis was performed to investigate the relationship between hCK-a with IHD. Parametric test was performed using independent t-test meanwhile non-parametric test was performed using Mann-Whitney test. As anticipated, CRP was found to be significant higher in expression both in mRNA level and protein level (Independent t-test, p<0.001; Mann-Whitney test, p= 0.019). In this study, a significant lower level of hCK-a2 mRNA expression in IHD patients was revealed (Independent ttest, p=0.032). However, total hCK-a mRNA expression was found to have no significant different between IHD patients and healthy volunteers (Mann-Whitney test, p=0.705). Protein expression level of hCK-a was found to be significantly lower in lHD patients as compared to healthy volunteers (Mann-Whitney test, p=0.014). Albeit preliminary, this study found that there may be a profound relationship between hCK-a and IHD.
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Keywords
Heart disease