Utilisation Of Molecular Markers For Determining Management Strategies Of The Blood Cockle, Anadara Granosa
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Date
2011-07
Authors
Mohd Ghani, Farhana
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Publisher
Universiti Sains Malaysia
Abstract
A population genetic study of the blood cockle, Anadara granosa, on the west coast of Peninsular Malaysia was conducted to define the genetic structuring and determine temporal variation between year classes (2007/2008 and 2008/2009) of this species. In addition, novel microsatellite markers were developed. The population investigation was based on the Cytochrome Oxidase subunit I mitochondrial DNA (COI mtDNA) marker that had previously used to infer population structuring of adult A. granosa. The genetic survey was done on seven spat populations and six adult populations involving populations from north to south of the west coast Peninsula Malaysia. Populations were defined into three regions: north, central and south. DNA sequencing of 149 samples revealed significant genetic structure of the south populations from the rest (ФST = 0.217, P < 0.05). All populations exhibited high haplotype diversity (h = 0.563-0.933) but low nucleotide diversity (π = 0.001-0.013) indicating recent population expansion. Temporal analysis between year classes revealed no significant genetic structure between both cohorts (ФCT = 0.018, P = 0.709) signalling consistent larval dispersal pattern over time. This study has provided further insight on the historical demography, dispersal pattern and population dynamics of this species.
Development of microsatellite markers for the blood cockle successfully isolated 82 positive clones containing microsatellite repeats with the majority of these (91.4%) being dinucleotide repeats. Based on the levels of polymorphism and non-overlapping molecular weights, 8 markers were further utilised on selected population. Levels of polymorphism were found to be between Ho = 0.103-0.833 and He = 0.773-0.937. However, one null alleles and significant linkage disequilibria were detected for five primers. These markers could be useful for blood cockle population management, genome mapping and marker-assisted selection to provide future groundwork for fisheries and aquaculture management
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Utilisation of molecular markers for determining , management strategies of the blood cockle