KEBERKESANAN EKSTRAK KASAR DARIPADA POKOK LEMBAR (CURCULIGO LATIFOLIA DRY AND) KE ATAS YIS, CANDIDA ALBICANS

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Date
2002-10
Authors
SULAIMAN, SURA YA
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A preliminary screening of extract derived from Curculigo latifolia Dryand indicated positive results on bacteria and yeasts except for aqueous extract. Extracts from hexanol and methanol exhibited significant antimicrobial activities against yeasts and bacteria but not against fungi. The MIC results from hexanol extract showed significant results on bacteria, (Bacillus subtilis, Enterobacter aerogenes and Erwinia sp. and yeasts, Candida albicans). However, the inhibition zones produced was partial inhibitions. On the other hand, the MIC results from methanol extracts showed significant results on Bacillus subtilis, Staphylococcus aureus and Candida albicans and were ranged from 7.5 mg/ml - 12.5 mg/ml. Therefore, the subsequent investigations were only focused on bacteria (Bacillus subtilis and Staphylococcus aureus) and yeast (Candida albicans). The methanol's extracts mode of action on yeast (Candida albicans) and bacteria, (Staphylococcus aureus and Bacillus subtilis) were observed through the changes occurred in their growth. The growth of Candida albicans was completely inhibited when more than 12.5 mg/ml of root extract and 7.5 mg/ml of extract from stem and leaves, respectively were used. The growth of bacteria, Staphylococcus aureus was inhibited when more than 7.5 mg/ml of extract from root and 12.5 mg/ml extract from stem and leaves used. On the other hand, the growth of Bacillus subtilis was inhibited when more than 7.5 mg/ml of extract of root and leaves and 12.5 mg/ml from stem used. These findings were supported by the SEM and TEM micrographes, which showed various changes occurred on the structure and morphology of the extract treated cells. The cytotoxicity test of the extracts was carried out using brine shrimp lethality assay. It was found that all the extracts showed significant values of lethality concentration of 50 (LC 50). The extracts were then separated using a thin layer chromatography and antibacterial test. The results showed that the extracts couldn't be separated easily.
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KEBERKESANAN EKSTRAK KASAR DARIPADA POKOK
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