KEBERKESANAN EKSTRAK KASAR DARIPADA POKOK LEMBAR (CURCULIGO LATIFOLIA DRY AND) KE ATAS YIS, CANDIDA ALBICANS
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Date
2002-10
Authors
SULAIMAN, SURA YA
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Abstract
A preliminary screening of extract derived from Curculigo latifolia Dryand indicated
positive results on bacteria and yeasts except for aqueous extract. Extracts from
hexanol and methanol exhibited significant antimicrobial activities against yeasts and
bacteria but not against fungi. The MIC results from hexanol extract showed
significant results on bacteria, (Bacillus subtilis, Enterobacter aerogenes and Erwinia
sp. and yeasts, Candida albicans). However, the inhibition zones produced was partial
inhibitions. On the other hand, the MIC results from methanol extracts showed
significant results on Bacillus subtilis, Staphylococcus aureus and Candida albicans
and were ranged from 7.5 mg/ml - 12.5 mg/ml. Therefore, the subsequent
investigations were only focused on bacteria (Bacillus subtilis and Staphylococcus
aureus) and yeast (Candida albicans).
The methanol's extracts mode of action on yeast (Candida albicans) and bacteria,
(Staphylococcus aureus and Bacillus subtilis) were observed through the changes
occurred in their growth. The growth of Candida albicans was completely inhibited
when more than 12.5 mg/ml of root extract and 7.5 mg/ml of extract from stem and
leaves, respectively were used. The growth of bacteria, Staphylococcus aureus was
inhibited when more than 7.5 mg/ml of extract from root and 12.5 mg/ml extract from
stem and leaves used. On the other hand, the growth of Bacillus subtilis was inhibited
when more than 7.5 mg/ml of extract of root and leaves and 12.5 mg/ml from stem
used. These findings were supported by the SEM and TEM micrographes, which
showed various changes occurred on the structure and morphology of the extract
treated cells.
The cytotoxicity test of the extracts was carried out using brine shrimp lethality
assay. It was found that all the extracts showed significant values of lethality
concentration of 50 (LC 50). The extracts were then separated using a thin layer
chromatography and antibacterial test. The results showed that the extracts couldn't
be separated easily.
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KEBERKESANAN EKSTRAK KASAR DARIPADA POKOK