The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7)
| dc.contributor.author | Mohd Fisall, Umiey Fahietah | |
| dc.date.accessioned | 2020-06-30T07:58:30Z | |
| dc.date.available | 2020-06-30T07:58:30Z | |
| dc.date.issued | 2019-08 | |
| dc.description.abstract | Moringa oleifera (MO) is belonging to Moringaceae family. In Malaysia, MO was known as „Munggai‟ or „Kelor‟ tree. MO is well known for its therapeutic properties that possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of various degree of polarities on the cytotoxicity of breast cancer cell line (MCF-7), were studied. The cytotoxicity effect of 80 % methanol and its solvent fractions of Moringa oleifera leave (MOL) extract against MCF-7 cell were determined by using MTS assay. The normal breast cell line (MCF-10A) was used to compare with MCF-7 cell. The effective extract that gave the most cytotoxic effect on MCF-7 cell had been chosen. Among of all extracts, dichloromethane extract (DF-CME-MOL) had been selected accordingly to its lowest IC50 (5 μg/mL) and highest selectivity index (SI) (9.5). Then, the chosen extract (DF-CME-MOL) was proceed for further analysis. The phytochemical analysis was performed using DPPH, ABTS, Folin-Ciocalteu, aluminium chloride and GC-MS analysis. An antioxidant contents in DF-CME-MOL for DPPH and ABTS assay were 10.52±0.009 mg/g TEAC and 4.32±0.021 mg/g TEAC, respectively. While, total phenolic and flavonoid content were 34.96±0.006 mg/g GAE and 15.72±0.050 mg/g QUE, respectively. After that, compound analysis by GC-MS detected some potential compounds in DF-CME-MOL named; Phenol, 4-(methoxymethyl)-, 2-Methoxy-4-vinylphenol, | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/9772 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Moringa Oleifera | en_US |
| dc.subject | Breast Cancer | en_US |
| dc.title | The Effect Of Moringa Oleifera Leaf Extract On Cytotoxicity And Apoptosis Pathway In Breast Cancer Cell (Mcf-7) | en_US |
| dc.type | Thesis | en_US |
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