Determination Of Mesenchymal Stem Cell (Msc) Adhesion On Surface Modified With Ionic Polymers
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Date
2015-08
Authors
Samynazan, Oor Vasi
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Mesenchymal stem cell (MSC) adhesion is influenced by the chemical and physical properties of extracellular membrane (ECM). For example, the electrostatic property of cationic and anionic sodium salt within ECM influence cell adhesion as well. However, the adhesion properties of MSC on surfaces constituting a combination of cationic and anionic polymer remains unclear. To uncover the complexity of cell-polymer interaction in in vitro settings, the ionic surfaces interaction with MSC was studied. Cationic such as poly (allylamine hydrochloride) (PAH) and anionic such as poly (4-styresulfonic-acid-co-malleic-acid) (PSSCMA) are used in designing the surfaces with different charges. In this study, five glass coverslips with different gradient of surface charges prepared by coating coverslip with varying ratio of PAH to PSSCMA using spin coater. Unmodified glass coverslip surfaces were used as control. The wettability testing of the surface were done using a goniometer. Surface with 50:50 gradient shows the highest contact angle , thus resisting the cell adhesion on the surface. Then the effects of effects of different PAH:PSSCMA gradient on MSC adhesion were investigated. Herein, cell numbers, cell density and the mean cell area determine the cell adhesion and spreadiness on different surfaces. Surfaces of 75:25 (PAH:PSSCMA) gradient have 13 cells/mm2 and higher cell area while surfaces of 100:0 (PAH:PSSCMA) have 3 cells/mm2 and with lowest cell area. In addition, the distribution of the vinculin in cell were determined as well. Vinculin was highly expressed on the 75:25 (PAH:PSSCMA) surface gradient followed by 25:75 (PAH:PSSCMA) while surfaces of 50:50 (PAH:PSSCMA) and 0:100 (PAH:PSSCMA) have lowest vinculin expression. Overall, MSC adhesion on ionic polymer surfaces has showed well expression of vinculin and better cell spreadiness.
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Keywords
Mesenchymal stem cell (MSC)