A Preliminary Study On Blood Coagulation Activities Of Malaysian Mikania Cordata Leaves
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Date
2015-08
Authors
Wan Shaffee, Wan Norshazwani
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Existing anticoagulants and antifibrinolytic agents have been reported to have lifethreatening
side effects. Therefore, a search for novel agents of natural origin is demanded
nowadays. Mikania cordata also known as ‘selaput tunggul’ was traditionally used by
folks to treat various infections and diseases. In this study the aimed was to investigate the
effect of M. cordata in blood coagulation activities and to analyse the bioactive
compounds in the leaves extract which might have significant effects on coagulation
activities of the blood. The in vitro blood coagulation activities such as prothrombin time
(PT), activated partial thromboplastin time (APTT) and thrombin time (TT) assay were
measured on citrated plasma from healthy volunteer donors. The plasma was spiked with
different concentration of aqueous leaves extract (0.78, 1.56, 3.13, 6.25, 12.50 and 25.00
mg/mL) and the results showed that the prolongation of APTT and TT in concentrationdependent
manner (p<0.01). However in the PT assay, the results showed procoagulant
activities at concentration 3.13 mg/mL and anticoagulant activities at concentration 12.5
and 25.0 mg/mL. The result of gas chromatography mass spectrometry (GCMS) analysis
showed the presence of bioactive compounds in aqueous extract of M. cordata such as
caffeic acids, bis (trimethylsilyl) ester O–(trimethylsilyl)- malic acid; 2,3,4,5- tetrakis-O-
(trimethylsilyl)- arabinose; 1,2,3,5- tetrakis-O- (trimethylsilyl)- arabinofuranose; octakis
(trimethylsilyl) melibiose; and octakis (trimethylsilyl)- maltose are known contributing
compounds for anticoagulant effects. As a conclusion, these findings suggest that M.
cordata may provide a potential plant based anticoagulant products for future clinical use.
Description
Keywords
Activities of the blood