Antimicrobial Activity Of Plant Extracts And Isolation And Characterization Of Alkaloids From Samanea Saman (Jacq.) Merr. Leaves
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Date
2011-06
Authors
Ajam, Sundis Mahdi Sahib
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Publisher
Universiti Sains Malaysia
Abstract
Due to the harmful effects on the occupants and environment, residual toxicity, and carcinogenic nature of synthetic biocides, plant-based biocides may offer safer alternatives for controlling bacterial and fungal bioaerosols that cause sick building syndromes and building deterioration. Therefore, the purpose of this study was to screen local flora for antimicrobial activity against bacteria and fungi isolated from sick enclosed buildings, fractionate and identify the compounds with the highest antimicrobial activities from the methanolic (80% MeOH) extract of Samanea saman leaves. Basically, the present study was divided into two parts. Part I involved the screening of 104 plants extracts obtained from 75 different plant species collected around the State of Penang. Part II concentrated on the isolation and characterization of alkaloids of the leaves of S. saman. The extracts were obtained from different parts of the plants in 80% MeOH. Antimicrobial activity was conducted by using the disk-diffusion method against one species of bacteria (Bacillus subtilis) and four filamentous fungi (Aspergillus flavus, A. niger, Penicillium oxalicum, and Cladosporium oxysporum) isolated from sick enclosed buildings in Penang Island. In the tested concentration of 100 mg/ml, 33 (31.3%) plant extracts showed antibacterial and 2 (1.9%) of them exhibited antifungal activities. Minimum inhibitory concentration (MIC) was determined for plant extracts that recorded antimicrobial activities. Among 104 plant materials screened, the methanolic extracts of S. saman leaves and Garcinia mangostana pericarp showed the most promising antibacterial activity inhibiting B. subtilis with an MIC of 0.078 mg/ml, whereas Cinnamomum zeylanicum bark extract was the most active against fungi with MICs values in the range of from 1.25 - 2.5 mg/ml.
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Keywords
Antimicrobial activity of plant extracts , alkaloids from Samanea saman