Publication: The detection of multidrug-resistant klebsiella pneumoniae using polymerase chain reaction
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Date
2025-01
Authors
Faruque, Mohammad Arafatuddin
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Abstract
Multidrug-resistant Klebsiella pneumoniae (MDR-Kp) is a major worldwide medical concern, particularly in healthcare settings because it is resistant to multiple antibiotics. This resistance is caused by enzymes such as beta-lactamases and carbapenems, which limit treatment options and increase mortality rates. Polymerase chain reaction (PCR) was used in this study as a fast and accurate way to detect genetic markers for bacterial identification and resistance genes. The study aimed to use PCR for detection of 16S ribosomal RNA (16sRNA), beta-lactamase TEM (blaTEM), cefotaximase-M-1(CTXM-1) and New Delhi metallo-beta-lactamase 1(NDM-1) in K. pneumoniae. Archived samples were collected from the INFORMM Biobank, cultured under aseptic conditions and their DNA was extracted using the boiling method. PCR was performed with specific primers and 2% agarose gel electrophoresis was used to visualize the amplified products. The results showed successful amplification of 16sRNA gene (100%) in all isolates confirming the bacterial identity. The blaTEM and CTXM-1 genes were detected in 78.9% and 89.4% of K. pnuemoniae isolates respectively, while non-specific bands were observed during NDM-1 amplification for majority (69%) of the isolates. The study demonstrates that PCR is an effective method for rapidly identifying MDP-Kp and its resistance genes
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