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The detection of multidrug-resistant klebsiella pneumoniae using polymerase chain reaction

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dc.contributor.authorFaruque, Mohammad Arafatuddin
dc.date.accessioned2025-12-08T03:42:01Z
dc.date.available2025-12-08T03:42:01Z
dc.date.issued2025-01
dc.description.abstractMultidrug-resistant Klebsiella pneumoniae (MDR-Kp) is a major worldwide medical concern, particularly in healthcare settings because it is resistant to multiple antibiotics. This resistance is caused by enzymes such as beta-lactamases and carbapenems, which limit treatment options and increase mortality rates. Polymerase chain reaction (PCR) was used in this study as a fast and accurate way to detect genetic markers for bacterial identification and resistance genes. The study aimed to use PCR for detection of 16S ribosomal RNA (16sRNA), beta-lactamase TEM (blaTEM), cefotaximase-M-1(CTXM-1) and New Delhi metallo-beta-lactamase 1(NDM-1) in K. pneumoniae. Archived samples were collected from the INFORMM Biobank, cultured under aseptic conditions and their DNA was extracted using the boiling method. PCR was performed with specific primers and 2% agarose gel electrophoresis was used to visualize the amplified products. The results showed successful amplification of 16sRNA gene (100%) in all isolates confirming the bacterial identity. The blaTEM and CTXM-1 genes were detected in 78.9% and 89.4% of K. pnuemoniae isolates respectively, while non-specific bands were observed during NDM-1 amplification for majority (69%) of the isolates. The study demonstrates that PCR is an effective method for rapidly identifying MDP-Kp and its resistance genes
dc.identifier.urihttps://erepo.usm.my/handle/123456789/23321
dc.language.isoen
dc.subject-
dc.titleThe detection of multidrug-resistant klebsiella pneumoniae using polymerase chain reaction
dc.typeResource Types::text::thesis::bachelor thesis
dspace.entity.typePublication
oairecerif.author.affiliationUniversiti Sains Malaysia
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