Publication: Characterizations, Antigenicity, And Immunogenicity Of Extracellular Vesicle Proteins From Iron-Deprived Mycolicibacterium Smegmatis
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Date
2025-09
Authors
Jayaram, Mehalene
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Abstract
Tuberculosis (TB), the world's leading infectious killer, remains a major global health challenge. BCG, the only available vaccine, provides limited protection that wanes over time. Extracellular vesicles (EVs) from Mycobacterium tuberculosis (MTB) have shown vaccine potential, but their isolation is hindered by slow bacterial growth and biohazard risks. This study used Mycolicibacterium smegmatis (MSMEG), a fast-growing, non-pathogenic model organism, to produce EVs under iron-depleted conditions. These EVs were evaluated for stability, antigenicity using three pooled TB positive patient sera and their potential as a BCG booster vaccine in mice. Lyophilized EVs maintained antigenicity and structural stability, as confirmed by nanoparticle tracking analysis, transmission electron microscopy and western blotting.
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Keywords
Characterizations , Antigenicity , And Immunogenicity Of Extracellular Vesicle Proteins , Iron-Deprived Mycolicibacterium Smegmatis