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Characterizations, Antigenicity, And Immunogenicity Of Extracellular Vesicle Proteins From Iron-Deprived Mycolicibacterium Smegmatis

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dc.contributor.authorJayaram, Mehalene
dc.date.accessioned2026-07-21T07:19:59Z
dc.date.available2026-07-21T07:19:59Z
dc.date.issued2025-09
dc.description.abstractTuberculosis (TB), the world's leading infectious killer, remains a major global health challenge. BCG, the only available vaccine, provides limited protection that wanes over time. Extracellular vesicles (EVs) from Mycobacterium tuberculosis (MTB) have shown vaccine potential, but their isolation is hindered by slow bacterial growth and biohazard risks. This study used Mycolicibacterium smegmatis (MSMEG), a fast-growing, non-pathogenic model organism, to produce EVs under iron-depleted conditions. These EVs were evaluated for stability, antigenicity using three pooled TB positive patient sera and their potential as a BCG booster vaccine in mice. Lyophilized EVs maintained antigenicity and structural stability, as confirmed by nanoparticle tracking analysis, transmission electron microscopy and western blotting.
dc.identifier.urihttps://erepo.usm.my/handle/123456789/24748
dc.language.isoen
dc.subjectCharacterizations
dc.subjectAntigenicity
dc.subjectAnd Immunogenicity Of Extracellular Vesicle Proteins
dc.subjectIron-Deprived Mycolicibacterium Smegmatis
dc.titleCharacterizations, Antigenicity, And Immunogenicity Of Extracellular Vesicle Proteins From Iron-Deprived Mycolicibacterium Smegmatis
dc.typeResource Types::text::thesis::doctoral thesis
dspace.entity.typePublication
oairecerif.author.affiliationUniversiti Sains Malaysia
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