Publication: Comparative Analysis Of Cysts And Trophozoites Of Giardia Duodenalis Using Proteomic And Bioinformatic Analysis
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Date
2025-05
Authors
Aziz, Ahmad Fudail Eiyad
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Abstract
Giardia duodenalis is a protozoan parasite responsible for giardiasis, a widespread gastrointestinal disease that significantly affects public health, particularly in developing regions. The parasite alternates between the motile trophozoite and the cyst stage, with transmission primarily occurring through contaminated water. Giardiasis causes symptoms such as diarrhea, malabsorption, and weight loss, severely impacting children’s health. Current diagnostic methods lack sensitivity and specificity, and treatment options are complicated by emerging drug resistance. This study aims to provide a comprehensive proteomic analysis of g. Duodenalis trophozoites and cysts, focusing on identifying key proteins and pathways for improving diagnostic approaches particulary for antigen tests by narrowing notable proteins that highly involved in the life cycle of g. Duodenalis through proteomic and bioinformatics analyses. Protein extraction methods (sds-lysis, urea-lysis, sonication, and rapigest-aided sonication) were optimized, with urea-lysis yielding the high protein concentrations and better protein separation. Mass spectrometry identified 712 non-redundant proteins, with 145 unique to trophozoites, 156 unique to cysts, and 57.7% common to both stages.
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Keywords
Comparative Analysis Cysts Trophozoites Giardia Duodenalis