A Genomic Analysis Of Paenibacillus macerans ATCC 8244, A Gram Positive Nitrogen Fixing Bacterium
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Date
2016-09
Authors
Abdul Rahman, Ahmad Yamin
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Abstract
Paenibacillus macerans ATCC 8244 is a Gram-positive, spore-forming
bacterium with possibly the widest metabolic capabilities among the Paenibacillus
genus. It is able to perform fermentation of hexoses, deoxyhexoses, pentoses,
cellulose, and hemicelluloses. In addition, its ability to fix nitrogen creates an
agronomic potential. This study presents a whole genomic approach to give a
holistic view of the features of this microorganism. A phylogenomic analysis
revealed that the nearest genome identified was Paenibacillus macerans strain
ZY18 with 98 percent identity. The draft genome assembly yielded a total of
7,305,296 base pairs (bp) formed by 97 scaffolds. The N50 value of the scaffold was
157,355 bp. The average scaffold size was 75,312 bp and the average GC ratio of the
draft genome was 53.14 %. Gene prediction and annotation performed at the scaffold
level yielded 6953 coding sequences. These included genes for 85 tRNAs and 9
rRNAs. A Clusters of Orthologous Groups (COGs) classification analysis revealed
that the highest number of coding sequences involved the “Carbohydrate Transport
and Metabolism” category with a total of 845 gene models. The draft genome of P.
macerans ATCC 8244 revealed 15 genes that are involved in nitrogen fixation. The
presence of genes involved in Bisphenol A (BPA) degradation showed a potential in
degrading this environmentally hazardous chemical. In addition, it has the capability
to produce 2-3 butanediol making it as a prospective producer of biofuel.
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A phylogenomic analysis revealed that the nearest genome identified was , Paenibacillus macerans strain ZY18 with 98 percent identity.