A Genomic Analysis Of Paenibacillus macerans ATCC 8244, A Gram Positive Nitrogen Fixing Bacterium

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dc.contributor.authorAbdul Rahman, Ahmad Yamin
dc.date.accessioned2017-01-18T06:38:17Z
dc.date.available2017-01-18T06:38:17Z
dc.date.issued2016-09
dc.description.abstractPaenibacillus macerans ATCC 8244 is a Gram-positive, spore-forming bacterium with possibly the widest metabolic capabilities among the Paenibacillus genus. It is able to perform fermentation of hexoses, deoxyhexoses, pentoses, cellulose, and hemicelluloses. In addition, its ability to fix nitrogen creates an agronomic potential. This study presents a whole genomic approach to give a holistic view of the features of this microorganism. A phylogenomic analysis revealed that the nearest genome identified was Paenibacillus macerans strain ZY18 with 98 percent identity. The draft genome assembly yielded a total of 7,305,296 base pairs (bp) formed by 97 scaffolds. The N50 value of the scaffold was 157,355 bp. The average scaffold size was 75,312 bp and the average GC ratio of the draft genome was 53.14 %. Gene prediction and annotation performed at the scaffold level yielded 6953 coding sequences. These included genes for 85 tRNAs and 9 rRNAs. A Clusters of Orthologous Groups (COGs) classification analysis revealed that the highest number of coding sequences involved the “Carbohydrate Transport and Metabolism” category with a total of 845 gene models. The draft genome of P. macerans ATCC 8244 revealed 15 genes that are involved in nitrogen fixation. The presence of genes involved in Bisphenol A (BPA) degradation showed a potential in degrading this environmentally hazardous chemical. In addition, it has the capability to produce 2-3 butanediol making it as a prospective producer of biofuel.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3486
dc.subjectA phylogenomic analysis revealed that the nearest genome identified wasen_US
dc.subjectPaenibacillus macerans strain ZY18 with 98 percent identity.en_US
dc.titleA Genomic Analysis Of Paenibacillus macerans ATCC 8244, A Gram Positive Nitrogen Fixing Bacteriumen_US
dc.typeThesisen_US
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