Utilization Of Palm Oil-Based By-Products And Waste As Feedstock For Polyhydroxyalkanoate Biosynthesis
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Date
2009-12
Authors
Kek, Yik Kang
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The utilization of by-products and wastes from the palm oil industry is aimed to
reduce the overall production cost of polyhydroxyalkanoate (PHA). In this study, palm
oil refining by-products; palm acid oil (PAO), palm kernel acid oil (PKAO), palm fatty
acid distillate (PFAD), and palm oil-based used cooking oil (UCO) were evaluated as
the carbon substrate for poly(3-hydroxybutyrate) [P(3HB)] and poly(3-
hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] synthesis using
Cupriavidus necator H16 and its transformant. Important cultural parameters that
significantly affected the cell biomass and P(3HB) synthesis of C. necator H16 grown
on 5 g/L PKAO were identified. An initial urea concentration of 4 mM (C/N ratio~38)
gave the highest DCW and total P(3HB); 5.4 and 3.2 g/L respectively. Elevation of
culture volume from 50 to 100 mL (in 250 mL flask) further enhanced the P(3HB)
production up to 77 wt% of DCW, whereby P(3HB) concentration of 4.1 g/L was
obtained from 5 g/L PKAO.
Description
Keywords
Palm Oil-Based By-Products And Waste , Feedstock For Polyhydroxyalkanoate Biosynthesis