Utilization Of Palm Oil-Based By-Products And Waste As Feedstock For Polyhydroxyalkanoate Biosynthesis

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dc.contributor.authorKek, Yik Kang
dc.date.accessioned2018-08-23T01:24:16Z
dc.date.available2018-08-23T01:24:16Z
dc.date.issued2009-12
dc.description.abstractThe utilization of by-products and wastes from the palm oil industry is aimed to reduce the overall production cost of polyhydroxyalkanoate (PHA). In this study, palm oil refining by-products; palm acid oil (PAO), palm kernel acid oil (PKAO), palm fatty acid distillate (PFAD), and palm oil-based used cooking oil (UCO) were evaluated as the carbon substrate for poly(3-hydroxybutyrate) [P(3HB)] and poly(3- hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] synthesis using Cupriavidus necator H16 and its transformant. Important cultural parameters that significantly affected the cell biomass and P(3HB) synthesis of C. necator H16 grown on 5 g/L PKAO were identified. An initial urea concentration of 4 mM (C/N ratio~38) gave the highest DCW and total P(3HB); 5.4 and 3.2 g/L respectively. Elevation of culture volume from 50 to 100 mL (in 250 mL flask) further enhanced the P(3HB) production up to 77 wt% of DCW, whereby P(3HB) concentration of 4.1 g/L was obtained from 5 g/L PKAO.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6381
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectPalm Oil-Based By-Products And Wasteen_US
dc.subjectFeedstock For Polyhydroxyalkanoate Biosynthesisen_US
dc.titleUtilization Of Palm Oil-Based By-Products And Waste As Feedstock For Polyhydroxyalkanoate Biosynthesisen_US
dc.typeThesisen_US
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