Aktiviti antimikrob ekstrak vernonia cinerea l., tridax procumbens l., dan emilia sonchifo/ia l. daripada famili asteraceae
| dc.contributor.author | Lachimanan, Yoga Latha (A/P) | |
| dc.date.accessioned | 2015-07-30T05:13:57Z | |
| dc.date.available | 2015-07-30T05:13:57Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | This research was conducted to study the antimicrobial effect of three plant extracts of Asteraceae family namely Vernonia cinerea L., Tridax procumbens L., and Emilia sonchifolia L. Fifteen types of extracts were extracted from V. cinerea, T. procumbens and E. Sonchifolia namely methanol, chloroform, diethyl ether, ethyl acetate, and buthanol. Screening of these different extracts for antimicrobial activity showed that the extracts possess significant antimicrobial activity against Gram positive bacteria, Gram negative bacteria and yeast cells but not against the fungi tested. The minimum inhibitory co ncentration (MIC) and the minimum lethal concentration (MLC) values for different extract of V. cinerea, T. procumbens dan E. sonchifolia had been determined in the range of 1.56 - 50.00 mg/ml and 3.13 - 100.00 mg/ml, respectively. The results of the screening study and the effects of concentration of the different extracts on microorganism revealed that the methanolic extract of V. cinerea exhibited most significant antimicrobial activity among the three plants. Hence, further study was concentrated only on methanol extract of V. cinerea. Methanol extract of V. cinerea at the MIC, 1/2 MIC and 2 times MIC concentration can inhibit the log phase of Candida albicans and Pseudomonas aeruginosa. Microscopic studies showed that the methanol extract of V. cinerea caused some physiological and morphological changes in the treated cells of C. albicans and P. aeruginosa. Based on scanning electron microscope (SEM) and transmission electron microscope (TEM) studies, the methanol extract of V. cinerea (100.00 mg/ml) exhibited two different types of mechanisms on the tested microorganisms such as attacking the cell wall and disrupting their metabolism. The toxicity test for methanol extract of V. cinerea was carried out using brine shrimp lethality test and mice acute toxicity test. The methanol extract of V. cinerea screened for toxicity against the brine shrimp had lethality concentration of 50% (LC50) value more than 1 mg/ml and screening for acute toxicity in mice had lethality dose of 50% (LD50) value more than 2000 mg/ml. These findings confirmed that the crude extract of V. cinerea was not toxic. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1039 | |
| dc.language.iso | other | en_US |
| dc.subject | Tridax procumbens | en_US |
| dc.subject | Famili asteraceae | en_US |
| dc.title | Aktiviti antimikrob ekstrak vernonia cinerea l., tridax procumbens l., dan emilia sonchifo/ia l. daripada famili asteraceae | en_US |
| dc.type | Thesis | en_US |
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