Micropropagation, Establishment Of Cell Suspension Cultures And Evaluation Of Antioxidant Activities Of Gynura Procumbens
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Date
2011-07
Authors
Pan, Lay Pin
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Aseptic bud explants of Gynura procumbens, obtained from the axillary buds of
field grown mature plants, could be established via surface-sterilization with 20 %
Clorox® for 20 minutes. The formation of multiple shoots could be induced on MS
medium supplemented with 2.0 mg/L BA with an average of 14.7 ± 0.7 shoots
produced from each bud explant after four weeks of culture. When the concentration
of BA supplemented into the MS medium was reduced to 1.5 mg/L BA, reasonably
high number of shoots (13.9 ± 1.3 shoots per explant) can still be produced with less
production of abnormal shoots. The optimum subculture cycle was four weeks. All
the four weeks old micro shoots produced roots when cultured on gelled basic MS
medium after six days of culture. All the in vitro plantlets survived after
acclimatization in the plant house. MS medium supplemented with 2 mg/L
Picloram was the best medium for induction of callus from the leaf explants. The
induced calli proliferated well on MS medium supplemented with 0.5 mg/L
Picloram with the average biomass of 7.874 ± 0.413 g after four weeks of culture.
Throughout the fourteen subculture cycles, the production of callus in term of
biomass was found to become constant after the 4th subculture cycle. The calli
produced were light yellow and friable in nature. Cell suspension culture could be
established by inoculating 0.5 g of this friable callus in 20 mL of MS medium
supplemented with 0.5 mg/L Picloram and 60 g/L sucrose. The cells proliferated
well under continuous light with light intensity of 2000 - 2500 lux. The methanolic
extracts of the cultured cells showed higher total flavonoid content, total phenolic
content and free radical scavenging activity compared to the mother plants, tissuecultured
plantlets and calli.
Description
Keywords
Micropropagation, establishment of cell suspension cultures , and evaluation of antioxidant activities of gynura procumbens