Micropropagation, Establishment Of Cell Suspension Cultures And Evaluation Of Antioxidant Activities Of Gynura Procumbens
| dc.contributor.author | Pan, Lay Pin | |
| dc.date.accessioned | 2018-09-06T01:58:14Z | |
| dc.date.available | 2018-09-06T01:58:14Z | |
| dc.date.issued | 2011-07 | |
| dc.description.abstract | Aseptic bud explants of Gynura procumbens, obtained from the axillary buds of field grown mature plants, could be established via surface-sterilization with 20 % Clorox® for 20 minutes. The formation of multiple shoots could be induced on MS medium supplemented with 2.0 mg/L BA with an average of 14.7 ± 0.7 shoots produced from each bud explant after four weeks of culture. When the concentration of BA supplemented into the MS medium was reduced to 1.5 mg/L BA, reasonably high number of shoots (13.9 ± 1.3 shoots per explant) can still be produced with less production of abnormal shoots. The optimum subculture cycle was four weeks. All the four weeks old micro shoots produced roots when cultured on gelled basic MS medium after six days of culture. All the in vitro plantlets survived after acclimatization in the plant house. MS medium supplemented with 2 mg/L Picloram was the best medium for induction of callus from the leaf explants. The induced calli proliferated well on MS medium supplemented with 0.5 mg/L Picloram with the average biomass of 7.874 ± 0.413 g after four weeks of culture. Throughout the fourteen subculture cycles, the production of callus in term of biomass was found to become constant after the 4th subculture cycle. The calli produced were light yellow and friable in nature. Cell suspension culture could be established by inoculating 0.5 g of this friable callus in 20 mL of MS medium supplemented with 0.5 mg/L Picloram and 60 g/L sucrose. The cells proliferated well under continuous light with light intensity of 2000 - 2500 lux. The methanolic extracts of the cultured cells showed higher total flavonoid content, total phenolic content and free radical scavenging activity compared to the mother plants, tissuecultured plantlets and calli. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/6516 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Micropropagation, establishment of cell suspension cultures | en_US |
| dc.subject | and evaluation of antioxidant activities of gynura procumbens | en_US |
| dc.title | Micropropagation, Establishment Of Cell Suspension Cultures And Evaluation Of Antioxidant Activities Of Gynura Procumbens | en_US |
| dc.type | Thesis | en_US |