Assessment of genetic relatedness among hydrilla verticillata (l. f.) royle accessions in malaysia using rapd and aflp markers.
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Date
2010
Authors
Choke Lin, Chin
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Abstract
Genetic relationships among 119 accessions of Hydrilla verticillata from 28 locations in
Malaysia were determined using Random Amplified Polymorphic DNA (RAPD) and 82
accessions of H verticillata from 26 locations were studied using the Amplified Fragment
Length Polymorphism (AFLP) technique. A total of nine RAPD primers produced 143
amplified fragments with 1 OS of them were polymorphic. RAPD data used to generate a
Neighbour-Joining tree and Principal Coordinate Analysis (PCO) clustered these accessions into
four groups that did not separate them according to geographical areas. A total of 326
polymorphic bands were generated using the AFLP technique with two primers combination.
The Neighbour-Joining tree produced from the AFLP data divided the accessions into four
groups that closely resemble those from RAPD analysis. However, the PCO results is
dissimilar with Neighbour-Joining in which only three groups were recognized. Overall the
level of genetic variation among accessions within a location is lower as compared to between
accessions from different locations. This shows that it is important to understand the
reproduction of the species within a location.
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Keywords
Genetic relatedness among , Aflp markers.