Assessment of genetic relatedness among hydrilla verticillata (l. f.) royle accessions in malaysia using rapd and aflp markers.

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dc.contributor.authorChoke Lin, Chin
dc.date.accessioned2015-07-30T04:35:20Z
dc.date.available2015-07-30T04:35:20Z
dc.date.issued2010
dc.description.abstractGenetic relationships among 119 accessions of Hydrilla verticillata from 28 locations in Malaysia were determined using Random Amplified Polymorphic DNA (RAPD) and 82 accessions of H verticillata from 26 locations were studied using the Amplified Fragment Length Polymorphism (AFLP) technique. A total of nine RAPD primers produced 143 amplified fragments with 1 OS of them were polymorphic. RAPD data used to generate a Neighbour-Joining tree and Principal Coordinate Analysis (PCO) clustered these accessions into four groups that did not separate them according to geographical areas. A total of 326 polymorphic bands were generated using the AFLP technique with two primers combination. The Neighbour-Joining tree produced from the AFLP data divided the accessions into four groups that closely resemble those from RAPD analysis. However, the PCO results is dissimilar with Neighbour-Joining in which only three groups were recognized. Overall the level of genetic variation among accessions within a location is lower as compared to between accessions from different locations. This shows that it is important to understand the reproduction of the species within a location.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1008
dc.language.isoenen_US
dc.subjectGenetic relatedness amongen_US
dc.subjectAflp markers.en_US
dc.titleAssessment of genetic relatedness among hydrilla verticillata (l. f.) royle accessions in malaysia using rapd and aflp markers.en_US
dc.typeThesisen_US
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