Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants
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Date
2015-10
Authors
Lim, Ying Yu
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The anti-aging properties of extracts from four different plants, namely O. stamineus,
C. hirta, P. sarmentosum and C. caudatus were investigated. A simple extraction
method was carried out before further analysis. DPPH assay indicated that ethanol
extract of C. hirta showed the highest radical scavenging activities, with IC50
of 33.3
± 1.8 μg/ml whilst ethanol extract of P. sarmentosum showed the lowest radical
scavenging activities, with IC50 of 660.4 ± 14.4 μg/ml. Toxicity of plant extracts was
tested on adult Human Dermal Fibroblast (HDFa). O. stamineus and C. caudatus
were toxic to cells at the concentration of 100μg/ml. The effects of the plant extracts
on Type I pro-collagen production were tested and it was found that O. stamineus, C.
hirta and C. caudatus accelerated PICP expressions when tested at the concentrations
of 50, 100, 250 and 500ng/ml. For the cytoprotection effects of plant extracts at the
concentrations of 50, 100, 250 and 500ng/ml towards the sub-lethal dose of hydrogen
peroxide at 750μg/ml, only 50ng/ml of O. stamineus showed protection against the
oxidative stress. Plant extracts were further investigated by using C. elegans as the
model in vivo organism. The nematodes were irradiated with ultraviolet B for 3
minutes and treated with plant extracts at the concentrations of 1, 10 and 100μg/ml.
O. stamineus and P. sarmentosum at the concentration of 10μg/ml increased the
mean lifespan to 7.0 ± 0.3 and 6.9 ± 0.2 days respectively compared to 6.2 ± 0.1 days
for untreated nematodes. Liposome was used as a carrier to deliver O. stamineus to C.
elegans to study its efficacy in delivering consistent amounts of the extract to the
nematodes. Even though high encapsulation efficiency was obtained, the liposomal
formulation did not prolong the lifespan of the C. elegans. Therefore it is important to test the efficacy of plant extracts both in vivo and in vitro for anti-aging studies as
skin aging is a complex biological process.
Description
Keywords
Anti-Oxidant, Anti-Aging Properties