Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants

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dc.contributor.authorLim, Ying Yu
dc.date.accessioned2017-02-08T04:45:32Z
dc.date.available2017-02-08T04:45:32Z
dc.date.issued2015-10
dc.description.abstractThe anti-aging properties of extracts from four different plants, namely O. stamineus, C. hirta, P. sarmentosum and C. caudatus were investigated. A simple extraction method was carried out before further analysis. DPPH assay indicated that ethanol extract of C. hirta showed the highest radical scavenging activities, with IC50 of 33.3 ± 1.8 μg/ml whilst ethanol extract of P. sarmentosum showed the lowest radical scavenging activities, with IC50 of 660.4 ± 14.4 μg/ml. Toxicity of plant extracts was tested on adult Human Dermal Fibroblast (HDFa). O. stamineus and C. caudatus were toxic to cells at the concentration of 100μg/ml. The effects of the plant extracts on Type I pro-collagen production were tested and it was found that O. stamineus, C. hirta and C. caudatus accelerated PICP expressions when tested at the concentrations of 50, 100, 250 and 500ng/ml. For the cytoprotection effects of plant extracts at the concentrations of 50, 100, 250 and 500ng/ml towards the sub-lethal dose of hydrogen peroxide at 750μg/ml, only 50ng/ml of O. stamineus showed protection against the oxidative stress. Plant extracts were further investigated by using C. elegans as the model in vivo organism. The nematodes were irradiated with ultraviolet B for 3 minutes and treated with plant extracts at the concentrations of 1, 10 and 100μg/ml. O. stamineus and P. sarmentosum at the concentration of 10μg/ml increased the mean lifespan to 7.0 ± 0.3 and 6.9 ± 0.2 days respectively compared to 6.2 ± 0.1 days for untreated nematodes. Liposome was used as a carrier to deliver O. stamineus to C. elegans to study its efficacy in delivering consistent amounts of the extract to the nematodes. Even though high encapsulation efficiency was obtained, the liposomal formulation did not prolong the lifespan of the C. elegans. Therefore it is important to test the efficacy of plant extracts both in vivo and in vitro for anti-aging studies as skin aging is a complex biological process.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3693
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectAnti-Oxidant, Anti-Aging Propertiesen_US
dc.titleEvaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plantsen_US
dc.typeThesisen_US
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