Anti-implamatory activity of ageratum conyzoides linn. lantana camara linn. and psidium guajava linn. extracts.
| dc.contributor.author | Chong Vee, Tan | |
| dc.date.accessioned | 2015-07-31T04:05:16Z | |
| dc.date.available | 2015-07-31T04:05:16Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | In an attempt to seek for more potent and less side effects anti-inflammatory agent(s), activity guided fractionation was performed on Ageratum conyzoides Linn., Lantana camara Linn. and Psidium guajava Linn. extracts. The extracts were screened using animal model carrageenan induced hind paw oedema. The model was optimised first to find the best suspending agent for water insoluble antiinflammatory agent indomethacin/extracts and to determine the type of experimental animals most sensitive to indomethacin inhibition of oedema. Compound tragacanth (1%) was found to be the best suspending agents compared to carboxymethyl cellulose (CMC) (0.5%), tween 80 (1%),and tween 80/ ethanol/ water (5: 5: 90) (1%). The animals that gave the largest inhibition of carrageenan-induced oedema to indomethacin was in the order of female rat > male rat > male mice > female mice. The plant materials were serially extracted with different solvent e.g. petroleum ether, chloroform and methanol. The methanolic extract of L. camara leaves was found to give the best inhibition of oedema compared to other extracts. Therefore, the methanolic L. camara leaves extract was fractionated into petroleum ether, chloroform, ethyl acetate, butanol and aqueous fractions using solvent-solvent partition method. It was found that the chloroform fraction caused the strongest inhibition on female rat hind paw oedema. The choloroform fraction was further fractionated into four subtractions (CF1 to CF4) using column chromatography. Results showed that CF4 was the most effective in reducing the oedema induced by carrageenan in female rats. The effect of CF4 of L. camara Linn. was then examined on the oxytocin and acetylcholine induced contraction in isolated rat uterus preparation to investigate whether the anti-inflammatory activity is due to the inhibition of prostaglandins synthesis. Result showed that CF4 inhibited the contraction induced by both oxytocin and acetylcholine in non-competitive manner which suggests that the action of CF4 is non specific. The possible blockade of voltage-operated calcium channels (VOCCs) and intracellular calcium channels of CF4 fraction was investigated using isolated rat vas deferens preparation. Results obtained suggested that CF4 selectively blocks intracellular calcium channel rather than VOCCs. The phytochemical screening using thin layer chromatography was performed on the active fractions of Lantana camara to identify the classes of compounds present in this fraction. The result indicated that CF4 contains cardiac glycosides, bitter principles, saponins, terpenoids, and essential oil. This study concluded that the Lantana camara extracts are able to exert anti-inflammatory activity through blockage of the intracellular calcium channel. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1077 | |
| dc.language.iso | en | en_US |
| dc.subject | Conyzoides linn. | en_US |
| dc.subject | Psidium guajava linn. | en_US |
| dc.title | Anti-implamatory activity of ageratum conyzoides linn. lantana camara linn. and psidium guajava linn. extracts. | en_US |
| dc.type | Thesis | en_US |
Files
License bundle
1 - 1 of 1
Loading...
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: