Assesment On The Cytotoxicity, Apoptosis And Autophagy Properties Of Chemically Synthesised Silver Nanoparticles On Beas 2B Cells
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Date
2017
Authors
Mohd Raflee, Nur Afiqah
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Silver nanoparticles (AgNPs) has been shown to exhibit anti- microbial effect.
The exploitation of AgNPs has emerged in the recent years and may trigger
major health problem. The effect of AgNPs on the human normal lung cells
have not been studied. Here, we report on the chemically-synthesised
AgNPs-induced cytotoxicity in human normal lung cells. In this study,
chemically-synthesised AgNPs were evaluated on their cytotoxicity effects in
Beas 2b human normal lung cells by measuring the cell viability, mitochondrial
membrane potential, autophagy and apoptosis. The cell viability of
AgNPs-treated cells was found to decrease in time- and dose-dependent
manner. AgNPs also found to reduce the mitochondrial membrane potential of
Beas 2b cells, which is an early indicator for the activation of apoptosis and
mitophagy. Co-treatment of AgNPs with BEZ-235, a PI3K/mTOR inhibitor, has
also increased the percentage of autophagy activity in Beas 2b cells. Prolong
exposure of Beas 2b cells to AgNPs has also significantly increased the
number of total apoptotic cell death. Therefore, this study suggest that
exposure of chemically-synthesised AgNPs, particularly at higher doses with
prolong incubation period, has induced cytotoxic effects in Beas 2b human
normal lung cells.
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Keywords
Chemically-synthesised AgNPs-induced cytotoxicity , in human normal lung cells