Assesment On The Cytotoxicity, Apoptosis And Autophagy Properties Of Chemically Synthesised Silver Nanoparticles On Beas 2B Cells

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dc.contributor.authorMohd Raflee, Nur Afiqah
dc.date.accessioned2017-10-06T02:20:04Z
dc.date.available2017-10-06T02:20:04Z
dc.date.issued2017
dc.description.abstractSilver nanoparticles (AgNPs) has been shown to exhibit anti- microbial effect. The exploitation of AgNPs has emerged in the recent years and may trigger major health problem. The effect of AgNPs on the human normal lung cells have not been studied. Here, we report on the chemically-synthesised AgNPs-induced cytotoxicity in human normal lung cells. In this study, chemically-synthesised AgNPs were evaluated on their cytotoxicity effects in Beas 2b human normal lung cells by measuring the cell viability, mitochondrial membrane potential, autophagy and apoptosis. The cell viability of AgNPs-treated cells was found to decrease in time- and dose-dependent manner. AgNPs also found to reduce the mitochondrial membrane potential of Beas 2b cells, which is an early indicator for the activation of apoptosis and mitophagy. Co-treatment of AgNPs with BEZ-235, a PI3K/mTOR inhibitor, has also increased the percentage of autophagy activity in Beas 2b cells. Prolong exposure of Beas 2b cells to AgNPs has also significantly increased the number of total apoptotic cell death. Therefore, this study suggest that exposure of chemically-synthesised AgNPs, particularly at higher doses with prolong incubation period, has induced cytotoxic effects in Beas 2b human normal lung cells.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/4829
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectChemically-synthesised AgNPs-induced cytotoxicityen_US
dc.subjectin human normal lung cellsen_US
dc.titleAssesment On The Cytotoxicity, Apoptosis And Autophagy Properties Of Chemically Synthesised Silver Nanoparticles On Beas 2B Cellsen_US
dc.typeThesisen_US
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