Mikropropagasi tumbuhan ubatan spesies zingiberaceae
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Date
2002-05
Authors
Weng Hing, Thong
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Abstract
The production of aseptic tissue from Zingiberaceae plant could be increased
by sterilising the explant tissue with I 00 mg/1 mercury cloride for five minutes,
followed by Clorox® I5% for I5 minutes in the first stage and Clorox® I 00/o for I 0
minutes in the second stage. Zingiber officinale explants could produce the most
number of multiple shoots, 9.8 shoots per explant, when cultured in Murashige and ·
Skoog (MS) medium (I962) supplemented with 2.0 mgll Benzyladenine (BA) and 2.0
mgll Indole Butyric Acid (IDA). This optimum medium was also found to be suitable
for in vitro . propagation of other Zingiberaceae species namely Curcuma domestica,
Curcuma zedoaria, Kaempferia galanga, Alpinia galanga and A/pinia conchigera. All
these species could produce multiple shoots when cultured in this medium. For
Zingiber officinale, Kaempferia galanga, Alpinia galanga and Alpinia conchigera.
number of multiple shoots . formed in solid proliferative medium and liquid
proliferative medium was not significantly different while Curcuma domestica and
Curcuma .zedOaria produced more multiple shoots in liquid medium than solid
medium. The suitable period of subculturing for Zingiber officinale, Curcuma
domestica, Curcuma zedoaria and A/pinia conchigera was four weeks while the most
suitable subculturing period for Kaempferia ga/anga and Alpinia galanga was eight
weeks. Continuously subculturing of these species caused a decrease in the number of
multiple shoots formed. After acclimatization in the growth chamber for two weeks,
77-90% of the in vitro Zingiberaceae plantlets survived and all these plantlets grew
well in the field.
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Keywords
Tumbuhan ubatan , Spesies zingiberaceae