Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric
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Date
2020-09
Authors
Nazri, Nor Amalia
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
Abstract
In forensic casework, DNA recovery and the efficiency of DNA extraction method is
one of the important key for the forensic investigator to identify the individual involved
with the crime through the DNA analysis. This study therefore is intended to evaluate three
parameters of phenol chloroform extraction method from a tiny blood stain on fabric cotton
in order to find suitable condition that can give a high DNA recovery. Highly significant of
P value suggest that the selected parameters which is the incubation time, incubation
temperature and storage temperature have influenced the DNA recovery. All parameters
show accepted purity value at ratio A260/A280 and very low purity value for A260/A230
in all extracted samples which may be attributed by several factors. In summary, the
efficiency to dissolve DNA was found to be good when sample was stored at 56ᴼC based
on the tested method.
Description
Keywords
DNA