Anti-Cancer Studies Of Garcinia Mangostana L. And Syzygium Campanulatum Korth Towards Colorectal Carcinoma
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Date
2011-12
Authors
Aisha, Abdalrahim F. A.
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
This study aims to investigate the of the anti-angiogenic and anti-colon cancer activity
extracts and isolates of Syzygium campanulatum and Garcinia mangostana. And to
prepare G. mangostana toluene extract in nanoparticles and α-mangostin in solid
dispersions in order to improve their aqueous solubility. Phytochemical analysis of S.
campanulatum extracts reveals relatively high concentration of betulinic acid (BA) and
polyphenols. BA fraction from S. campanulatum showed potent cytotoxicity on colon
cancer cells by inducting the mitochondrial pathway of apoptosis. Extracts also showed
potent inhibition of angiogenesis in several in vitro and in vivo models, by inhibiting the
expression of vascular endothelial growth factor and inhibiting migration of human
endothelial cells. The methanolic extract also inhibits tumor growth in xenograft tumor
model with inhibition of intratumor blood vessels.
α- and γ-mangostin were isolated from G. mangostana fruit rinds, and were used to
standardize the G. mangostana extracts by UV spectrophotometric and HPLC methods.
The isolates and the xanthone extracts demonstrated potent cytotoxicity on colon cancer
cells by inducing the mitochondrial apoptosis pathway, and also showed effective in
vitro anti tumorigenicity. The compounds induced the transcription factor activity of
p53, Myc/Max and MAPK/ERK signaling pathways and inhibit the NF-KB pathway.
The toluene extract of G. mangostana showed significant delayed onset inhibition of
tumor growth in vivo. S. campanulatum and G. mangostana extracts did not show
severe toxic effects and the LD50 in Sprague Dawley rats was more than 2000 mg/kg
body weight. Cationic nanoparticles of G. mangostana toluene extract were prepared by
the nanoprecipitation method in Eudragit polymers RL100 and RS100. Particle size was
below 70 nm with high entrapment efficiency (>90%).
Description
Keywords
The anti-angiogenic and anti-colon cancer , isolates of Syzygium campanulatum