Morphological And Molecular Analysis Of Penaeus Semisulcatus From The Persian Gulf And Oman Sea

dc.contributor.author	Jahromi, Saeid Tamadoni
dc.date.accessioned	2018-08-02T01:15:09Z
dc.date.available	2018-08-02T01:15:09Z
dc.date.issued	2011-06
dc.description.abstract	There are two morphotypes of the green tiger shrimp, Penaeus semisulcatus recognized in the Persian Gulf and Oman Sea. The two morphotypes are distinguished by colour pattern of antenna and body transverse lines. This study is conducted to investigate the taxonomic status of the two morphotypes based on morphometrics and mitochondria DNA sequence analysis. In addition, population genetics study on P. semisulcatus from the Persian Gulf was performed to assist the selection of suitable broodstocks in future breeding programs. A total of 25 characters were investigated for morphometric study. Ten of these characters showed significant differentiation between single sex and combined sex (P < 0.05). Regression analysis showed that the correlation between the carapace length and body length of females in the non-banded morphotype, were different to those of the males. Molecular investigation of mitochondria DNA (mtDNA) using partial sequences of 16S rRNA gene showed relatively low genetic differences between the P. semisulcatus morphotypes with 3.9 % genetic distance. These sequences were able to distinguish between the two morphotypes, and separated them into two distinct clades. By contrast, genetic divergence detected by COI gene analysis was consistently higher. High genetic divergence for COI with 76 divergent sites was observed between the two morphotypes. Eight novel microsatellite loci were developed to study the population genetics structure of P. semisulcatus in three population sites in the Persian Gulf and Oman Sea (Jask, Hormoz and Kuhestak). There were incidences of heterozygosity deficiency and significant deviation from HWE showed at most loci (P <0.001). However, there were four loci (loci E2 and B9 – Hormoz; loci C6 – Kuhestak; and loci H9 - Jask) found to be in the Hardy Weinberg equilibrium. Micro-Checker analysis revealed of null alleles in the three microsatellite loci (B5, C6 and C9). Pairwise Fst comparison based on allelic and genotypic frequencies indicated that the three populations were significantly differentiated from each other (P <0.05). High levels of pairwise Fst (0.106) and low levels of Nm (2.103) observed between the Hormoz and Jask populations indicated restricted gene flow between the two populations.	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/6121
dc.language.iso	en	en_US
dc.publisher	Universiti Sains Malaysia	en_US
dc.subject	Morphological and molecular analysis of Penaeus semisulcatus	en_US
dc.subject	from the persian gulf and Oman sea	en_US
dc.title	Morphological And Molecular Analysis Of Penaeus Semisulcatus From The Persian Gulf And Oman Sea	en_US
dc.type	Thesis	en_US