MOLECULAR CHARACTERIZATION OF Fusarium oxysporum f. sp. cubense (FOe) IN MALAYSIA
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Date
2009-05
Authors
LEONG, SAU KUEEN
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Abstract
A total of 59 Fusarium oxysporum f. sp. cubense (FOC) isolates were analysed
using ERIC-PCR, RAMS and RFLP-IGS. The three molecular techniques showed
intraspecific variation among the FOC isolates, whereby 34 ERIC banding patterns,
33 IGS haplotypes and 13 - 26 micro satellite banding patterns were generated. The
banding patterns generated using each technique were highly variable, but showed
similarities among the FOC isolates. UPGMA cluster analyses based on ERIC-PCR
(15.4% - 100%) and RAMS (11.1% - 100%) showed similarity values very close to
those generated based on combined data (11.0% - 100%) of the three techniques,
while RFLP-IGS showed higher similarity values of approximately 60% - 100%
among the FOC isolates. Using similarity coefficient of SMC and lC, the clustering
of the isolates were slightly different when data from ERIC-PCR, RAMS and RFLPIGS
were analysed individually and collectively. Based on the dendrograms, most of
the FOC isolates including four FOC isolates from Indonesia were clustered in
clusters I and II regardless of the banana cultivars and locations. Two F oxysporum
isolates from the soil showed high genetic similarity and were always clustered
together with the FOC isolates. For two F solani isolates (angsana and pisang awak),
the banding patterns generated using the three molecular techniques were different
from those of the FOC isolates and were clustered separately from the FOC isolates.
Sequence analysis of the TEF-ln gene using parsimony and the distance methods
also showed intraspecific variations of the FOC isolates. The results supported the
hypothesis that the FOC isolates co-evolved with the edible bananas and their diploid
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Keywords
MOLECULAR , CHARACTERIZATION