MOLECULAR CHARACTERIZATION OF Fusarium oxysporum f. sp. cubense (FOe) IN MALAYSIA

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dc.contributor.authorLEONG, SAU KUEEN
dc.date.accessioned2016-01-12T03:28:19Z
dc.date.available2016-01-12T03:28:19Z
dc.date.issued2009-05
dc.description.abstractA total of 59 Fusarium oxysporum f. sp. cubense (FOC) isolates were analysed using ERIC-PCR, RAMS and RFLP-IGS. The three molecular techniques showed intraspecific variation among the FOC isolates, whereby 34 ERIC banding patterns, 33 IGS haplotypes and 13 - 26 micro satellite banding patterns were generated. The banding patterns generated using each technique were highly variable, but showed similarities among the FOC isolates. UPGMA cluster analyses based on ERIC-PCR (15.4% - 100%) and RAMS (11.1% - 100%) showed similarity values very close to those generated based on combined data (11.0% - 100%) of the three techniques, while RFLP-IGS showed higher similarity values of approximately 60% - 100% among the FOC isolates. Using similarity coefficient of SMC and lC, the clustering of the isolates were slightly different when data from ERIC-PCR, RAMS and RFLPIGS were analysed individually and collectively. Based on the dendrograms, most of the FOC isolates including four FOC isolates from Indonesia were clustered in clusters I and II regardless of the banana cultivars and locations. Two F oxysporum isolates from the soil showed high genetic similarity and were always clustered together with the FOC isolates. For two F solani isolates (angsana and pisang awak), the banding patterns generated using the three molecular techniques were different from those of the FOC isolates and were clustered separately from the FOC isolates. Sequence analysis of the TEF-ln gene using parsimony and the distance methods also showed intraspecific variations of the FOC isolates. The results supported the hypothesis that the FOC isolates co-evolved with the edible bananas and their diploiden_US
dc.identifier.urihttp://hdl.handle.net/123456789/1377
dc.subjectMOLECULARen_US
dc.subjectCHARACTERIZATIONen_US
dc.titleMOLECULAR CHARACTERIZATION OF Fusarium oxysporum f. sp. cubense (FOe) IN MALAYSIAen_US
dc.typeThesisen_US
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