POPULATION GENETIC STUDIES OF MARBLE GOBY Oxyeleotris marmoratus (BLEEKER, 1852) IN MALAYSIA USING MICROSATELLITE AND MITOCHONDRIAL DNA MARKERS
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Date
2008-06
Authors
ALI @ JAAFAR, RUZAINAH
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Abstract
Marble goby from 13 populations in Malaysia were genetically characterised
by examining the variability of 10 newly developed microsatellite loci coupled with
the nucleotide sequences of mitochondrial Cyt b and NO 5 genes. Microsatellite loci
isolated by the enrichment method of Random Amplified Microsatellite (RAM) were
evaluated for population variability and at least eight were found to be polymorphic
in all populations tested. A hierarchical AMOYA on microsatellite variability
showed significant population differentiation within the total sample of 13
populations but none for among group comparisons (FCT) were significant, i.e., no
genetic differentiation between the defined geographical regions; Borneo, West and
East Peninsular Malaysia. From the 176 individuals examined for the Cyt b gene, 16
unique haplotypes were generated and 57 unique ND 5 gene haplotypes were
identified. For all criteria of genetic variability (for example, polymorphic and
heterozygosity levels, nucleotide and haplotype diversities) the NO 5 gene analysis
had higher values. The heterogeneity in haplotype frequency distribution translated
into statistically significant FST value in all pairwise comparison for both genes.
Hierarchical analysis showed the partitioning of variation was almost similar among
each level for Cyt b gene. Structuring between populations within region was more
obvious for NO 5 gene. With the exception of the Borneo group, NJ, MP and MSN
analyses successfully cluster populations to their defined groups; the East and West
Peninsular regions. Population distribution is hypothesized to be influenced by
historical origin and geological factors. ]n general. the investigated populations still
retain healthy levels of variation In conclusion, molecular "markers, mtDNA and
microsatellites provided an efficient tool to identify variability and phylogenetic
relationships of the marble goby in Malaysia. Such studies is intended as a baseline
for future genetic monitoring and may contribute significantly in the development of
breeding programmes for production and management of the marble goby species in
Malaysia.
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Keywords
POPULATION GENETIC , MICROSATELLITE