POPULATION GENETIC STUDIES OF MARBLE GOBY Oxyeleotris marmoratus (BLEEKER, 1852) IN MALAYSIA USING MICROSATELLITE AND MITOCHONDRIAL DNA MARKERS
| dc.contributor.author | ALI @ JAAFAR, RUZAINAH | |
| dc.date.accessioned | 2016-01-12T03:41:39Z | |
| dc.date.available | 2016-01-12T03:41:39Z | |
| dc.date.issued | 2008-06 | |
| dc.description.abstract | Marble goby from 13 populations in Malaysia were genetically characterised by examining the variability of 10 newly developed microsatellite loci coupled with the nucleotide sequences of mitochondrial Cyt b and NO 5 genes. Microsatellite loci isolated by the enrichment method of Random Amplified Microsatellite (RAM) were evaluated for population variability and at least eight were found to be polymorphic in all populations tested. A hierarchical AMOYA on microsatellite variability showed significant population differentiation within the total sample of 13 populations but none for among group comparisons (FCT) were significant, i.e., no genetic differentiation between the defined geographical regions; Borneo, West and East Peninsular Malaysia. From the 176 individuals examined for the Cyt b gene, 16 unique haplotypes were generated and 57 unique ND 5 gene haplotypes were identified. For all criteria of genetic variability (for example, polymorphic and heterozygosity levels, nucleotide and haplotype diversities) the NO 5 gene analysis had higher values. The heterogeneity in haplotype frequency distribution translated into statistically significant FST value in all pairwise comparison for both genes. Hierarchical analysis showed the partitioning of variation was almost similar among each level for Cyt b gene. Structuring between populations within region was more obvious for NO 5 gene. With the exception of the Borneo group, NJ, MP and MSN analyses successfully cluster populations to their defined groups; the East and West Peninsular regions. Population distribution is hypothesized to be influenced by historical origin and geological factors. ]n general. the investigated populations still retain healthy levels of variation In conclusion, molecular "markers, mtDNA and microsatellites provided an efficient tool to identify variability and phylogenetic relationships of the marble goby in Malaysia. Such studies is intended as a baseline for future genetic monitoring and may contribute significantly in the development of breeding programmes for production and management of the marble goby species in Malaysia. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1438 | |
| dc.subject | POPULATION GENETIC | en_US |
| dc.subject | MICROSATELLITE | en_US |
| dc.title | POPULATION GENETIC STUDIES OF MARBLE GOBY Oxyeleotris marmoratus (BLEEKER, 1852) IN MALAYSIA USING MICROSATELLITE AND MITOCHONDRIAL DNA MARKERS | en_US |
| dc.type | Thesis | en_US |
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