Production Of Fructosyltransferase And Fructooligosaccharides By Penicillium Simplicissimum
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Date
2010-05
Authors
Yazid, Noraziah Abu
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Fructooligosaccharides (FOS) produced from natural resources as an
alternative sweetener has received greater attention and sucrose is the common
carbon source for its production. To minimize the production cost, feasibility of
using low cost substrates derived from local agricultural resources such as sugarcane
juice was chosen to replace the pure sucrose. Several fungi were screened for their
ability to produce fructosyltransferase (FTase) and FOS. Penicillium simplicissimum
was found to be a potent producer for FTase and FOS with the highest FTase activity
at 5.54 X 102 IU/ml and FOS yield per sucrose consumed of 0.39 g/g, respectively.
Fermentation was undertaken in batch mode using P. simplicissimum and
sugarcane juice as a substrate for the FOS production in shake flask culture. Several
parameters were studied using one-factor-at-a-time method and resulted that the
highest mycelial biomass and FOS production were at 10.07 g/L and 66.50 g/L,
accordingly. Due to the troublesome of filamentous fungal fermentation by freely
suspended mycelium, the morphology was controlled by mycelia immobilization in a
rotating fibrous bed bioreactor and showed good result with the highest mycelia
biomass and FOS productivity at 0.21 g/L·h and 4.01 g/L·h, respectively.
For optimization studies, the process parameters were further studied in shake
flasks and bioreactor using response surface methodology (RSM). Under optimum
conditions obtained from RSM in shake flasks culture, higher mycelial biomass were
produced at 7.43 g/L and FOS yield using extra- and intracellular FTase source at
0.83 g/g and 0.75 g/g, respectively. In bioreactor studies, satisfactory results were
obtained for the freely suspended and immobilized mycelia system with the
coefficient of determination, R2 for FOS yield higher than 0.9. Under optimum
conditions, the immobilized cells system predicted the highest FOS yield at 1.76 g/g.
Description
Keywords
Production of fructosyltransferase and fructooligosaccharides , by penicillium simplicissimum