Production Of Fructosyltransferase And Fructooligosaccharides By Penicillium Simplicissimum
| dc.contributor.author | Yazid, Noraziah Abu | |
| dc.date.accessioned | 2018-10-25T02:10:28Z | |
| dc.date.available | 2018-10-25T02:10:28Z | |
| dc.date.issued | 2010-05 | |
| dc.description.abstract | Fructooligosaccharides (FOS) produced from natural resources as an alternative sweetener has received greater attention and sucrose is the common carbon source for its production. To minimize the production cost, feasibility of using low cost substrates derived from local agricultural resources such as sugarcane juice was chosen to replace the pure sucrose. Several fungi were screened for their ability to produce fructosyltransferase (FTase) and FOS. Penicillium simplicissimum was found to be a potent producer for FTase and FOS with the highest FTase activity at 5.54 X 102 IU/ml and FOS yield per sucrose consumed of 0.39 g/g, respectively. Fermentation was undertaken in batch mode using P. simplicissimum and sugarcane juice as a substrate for the FOS production in shake flask culture. Several parameters were studied using one-factor-at-a-time method and resulted that the highest mycelial biomass and FOS production were at 10.07 g/L and 66.50 g/L, accordingly. Due to the troublesome of filamentous fungal fermentation by freely suspended mycelium, the morphology was controlled by mycelia immobilization in a rotating fibrous bed bioreactor and showed good result with the highest mycelia biomass and FOS productivity at 0.21 g/L·h and 4.01 g/L·h, respectively. For optimization studies, the process parameters were further studied in shake flasks and bioreactor using response surface methodology (RSM). Under optimum conditions obtained from RSM in shake flasks culture, higher mycelial biomass were produced at 7.43 g/L and FOS yield using extra- and intracellular FTase source at 0.83 g/g and 0.75 g/g, respectively. In bioreactor studies, satisfactory results were obtained for the freely suspended and immobilized mycelia system with the coefficient of determination, R2 for FOS yield higher than 0.9. Under optimum conditions, the immobilized cells system predicted the highest FOS yield at 1.76 g/g. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/6882 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Production of fructosyltransferase and fructooligosaccharides | en_US |
| dc.subject | by penicillium simplicissimum | en_US |
| dc.title | Production Of Fructosyltransferase And Fructooligosaccharides By Penicillium Simplicissimum | en_US |
| dc.type | Thesis | en_US |
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