Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(phenyl) methyl]-2-(2,4-dinitrophenyl)hydrazine Derivative On Cervical Cancer Cells
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Date
2016-09
Authors
Shaik Othuman, Faiqah Husna
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Cervical cancer remains as the second most common type of cancer in women and it
is the third leading cause of death despite various treatments that are available.
Inefficiency of present treatments had triggered scientists to seek for an alternative
treatment for this disease. Development of new anti-proliferative agent for cervical
cancer is an important innovation in fighting this miserable disease. Although, synthetic
hydrazone derivatives are widely being used in cancer research, the actual anticancer
mechanism shown by these chemicals is still not yet understood. Therefore this study
aimed to elucidate the anti-proliferative effects of the 1-[(Bromomethyl)(phenyl)methyl]
-2-(2,4 -dinitrophenyl) hydrazine (C14H11BrN4O4) derivative (Hydrazone compound) on
cervical cancer cells through determination of inhibitory concentration (IC50),
anti-proliferative assay, apoptosis assay, cell cycle assay, western blot analysis, invasion
assay and cytotoxicity analysis. The results showed the IC50 of the hydrazone compound
at 0.03mg/ml on the cervical cancer cells and inhibits the proliferation of cervical cancer
cells in a dose and time-dependent manner compared to the untreated control. In addition,
the flow cytometry analysis confirmed the hydrazone derivative causes S and G2/M
phase cell cycle arrest without induction of apoptosis. Western blot analysis showed that
hydrazone compound had increased the p27kip1 and PCNA and suppressed Cyclin E2 and
cdc25 protein expression. The anti-metastatic effect was studied through invasion assay
and it showed the hydrazone compound had inhibited 50.94% of HeLa cells invasion. It
is concluded the hydrazone compound possesses anti-proliferative effect on HeLa cells.
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Keywords
Investigation of the anti-proliferative effect , derivative on cervical cancer cells