Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(phenyl) methyl]-2-(2,4-dinitrophenyl)hydrazine Derivative On Cervical Cancer Cells

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dc.contributor.authorShaik Othuman, Faiqah Husna
dc.date.accessioned2019-07-12T07:39:22Z
dc.date.available2019-07-12T07:39:22Z
dc.date.issued2016-09
dc.description.abstractCervical cancer remains as the second most common type of cancer in women and it is the third leading cause of death despite various treatments that are available. Inefficiency of present treatments had triggered scientists to seek for an alternative treatment for this disease. Development of new anti-proliferative agent for cervical cancer is an important innovation in fighting this miserable disease. Although, synthetic hydrazone derivatives are widely being used in cancer research, the actual anticancer mechanism shown by these chemicals is still not yet understood. Therefore this study aimed to elucidate the anti-proliferative effects of the 1-[(Bromomethyl)(phenyl)methyl] -2-(2,4 -dinitrophenyl) hydrazine (C14H11BrN4O4) derivative (Hydrazone compound) on cervical cancer cells through determination of inhibitory concentration (IC50), anti-proliferative assay, apoptosis assay, cell cycle assay, western blot analysis, invasion assay and cytotoxicity analysis. The results showed the IC50 of the hydrazone compound at 0.03mg/ml on the cervical cancer cells and inhibits the proliferation of cervical cancer cells in a dose and time-dependent manner compared to the untreated control. In addition, the flow cytometry analysis confirmed the hydrazone derivative causes S and G2/M phase cell cycle arrest without induction of apoptosis. Western blot analysis showed that hydrazone compound had increased the p27kip1 and PCNA and suppressed Cyclin E2 and cdc25 protein expression. The anti-metastatic effect was studied through invasion assay and it showed the hydrazone compound had inhibited 50.94% of HeLa cells invasion. It is concluded the hydrazone compound possesses anti-proliferative effect on HeLa cells.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8474
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectInvestigation of the anti-proliferative effecten_US
dc.subjectderivative on cervical cancer cellsen_US
dc.titleInvestigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(phenyl) methyl]-2-(2,4-dinitrophenyl)hydrazine Derivative On Cervical Cancer Cellsen_US
dc.typeThesisen_US
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