Preparation And Characterization Of Xylo-Oligosaccharides From Oil Palm Frond (Elaeis Guineensis)
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Date
2011-07
Authors
Hussin, Farah Salina
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
This study focused on factors relevant for enzymatic hydrolysis of lignocellulosic
raw materials derived from oil palm frond (OPF). Two origins of xylanase enzyme
were used ; Trichoderma viride (T.viride xylanase) and Thermomyces lanuginosus
(T.lanuginosus xylanase) at its own optimum pH 5.5 and pH 6.5. The major interest
of this work is the isolation of xylo-oligosaccharides (XOS) from delignified
hemicellulose by using enzymatic hydrolysis method. Delignification of OPF was
done by alkaline peroxide treatment at 80 oC and the extraction of hemicellulose by
using alkaline extraction method. The results of Klason lignin showed a reduction of
lignin content from 24.17 % in OPF samples to 15.25 % in crude hemicellulose and
1.28 % in delignified hemicellulose. The maximum yield of XOS was determined
by comparing the conditions of both enzyme in terms of temperature (40 oC, 45 oC,
50 oC, 55 oC and 60 oC), time (10 hours of reaction), enzyme concentration
(0.05 U/ml, 1 U/ml, 2 U/ml, 3 U/ml, 4 U/ml and 5 U/ml) and also substrate
concentration (5 %, 10 %, 15 %, 20 % and 25 %). For T.viride xylanase, the
maximum yield of XOS were obtained at the seventh hour, 50 oC, 4 U/ml enzyme
and 10 % w/v substrate by yielding 57.6 % (2.42 g/kg of xylotriose and 3.34 g/kg of
xylobiose). As for T.lanuginosus xylanase, the maximum yield of XOS were
obtained at the third hour, 55 oC, 1 U/ml enzyme and 20 % w/v substrate by yielding
27.0 % (2.44 g/kg of xylotriose and 2.95 g/kg of xylobiose). Water binding
capacities showed significant differences with XOSTL showed higher water binding
capacity than XOSTV. XOSTL has a higher oil binding capacity than XOSTV. Data
also showed the calcium and magnesium binding capacity of XOS continued to
increase as the pH was increased. In solubility test, XOSTV was proven to be more
soluble than XOSTL with 88.70 % of solubility rate.
Description
Keywords
Characterization of xylo-oligosaccharides , from oil palm frond