Preparation And Characterization Of Xylo-Oligosaccharides From Oil Palm Frond (Elaeis Guineensis)

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dc.contributor.authorHussin, Farah Salina
dc.date.accessioned2018-08-28T08:05:00Z
dc.date.available2018-08-28T08:05:00Z
dc.date.issued2011-07
dc.description.abstractThis study focused on factors relevant for enzymatic hydrolysis of lignocellulosic raw materials derived from oil palm frond (OPF). Two origins of xylanase enzyme were used ; Trichoderma viride (T.viride xylanase) and Thermomyces lanuginosus (T.lanuginosus xylanase) at its own optimum pH 5.5 and pH 6.5. The major interest of this work is the isolation of xylo-oligosaccharides (XOS) from delignified hemicellulose by using enzymatic hydrolysis method. Delignification of OPF was done by alkaline peroxide treatment at 80 oC and the extraction of hemicellulose by using alkaline extraction method. The results of Klason lignin showed a reduction of lignin content from 24.17 % in OPF samples to 15.25 % in crude hemicellulose and 1.28 % in delignified hemicellulose. The maximum yield of XOS was determined by comparing the conditions of both enzyme in terms of temperature (40 oC, 45 oC, 50 oC, 55 oC and 60 oC), time (10 hours of reaction), enzyme concentration (0.05 U/ml, 1 U/ml, 2 U/ml, 3 U/ml, 4 U/ml and 5 U/ml) and also substrate concentration (5 %, 10 %, 15 %, 20 % and 25 %). For T.viride xylanase, the maximum yield of XOS were obtained at the seventh hour, 50 oC, 4 U/ml enzyme and 10 % w/v substrate by yielding 57.6 % (2.42 g/kg of xylotriose and 3.34 g/kg of xylobiose). As for T.lanuginosus xylanase, the maximum yield of XOS were obtained at the third hour, 55 oC, 1 U/ml enzyme and 20 % w/v substrate by yielding 27.0 % (2.44 g/kg of xylotriose and 2.95 g/kg of xylobiose). Water binding capacities showed significant differences with XOSTL showed higher water binding capacity than XOSTV. XOSTL has a higher oil binding capacity than XOSTV. Data also showed the calcium and magnesium binding capacity of XOS continued to increase as the pH was increased. In solubility test, XOSTV was proven to be more soluble than XOSTL with 88.70 % of solubility rate.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6441
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectCharacterization of xylo-oligosaccharidesen_US
dc.subjectfrom oil palm fronden_US
dc.titlePreparation And Characterization Of Xylo-Oligosaccharides From Oil Palm Frond (Elaeis Guineensis)en_US
dc.typeThesisen_US
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