Investigation of yb-1 gene expression by syber-green based real-time pcr in brain tumor.

Loading...
Thumbnail Image
Date
2008
Authors
Muhammad Redzwan, Sidik
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia.
Abstract
Cancer generally is a genetic disease that can cause alteration in specific gene in which a group of cells display uncontrolled growth, invasion and metastasis. YB-1 Gene Y -box binding protein (YB-1) is a member of the cold-shock domain (CSD) protein superfamily and involved in many cellular functions, including transcriptional regulation, translational regulation, DNA repair, drug resistance and stress responses to extracellular signals. YB-1 gene can be found in a cytoplasm and nuclear cell. The YB-1 gene comprises 8 exons spanning 19 kb of genomic DNA and is located on chromosome I p34. The aim of the study is to differential relatively quantitative the expression of YB-I gene in brain tumor sample (meningioma) and normal brain sample (Astrocyte SVG I2). SYBR-Green I based Real-Time PCR was performed and data analysis using formula 2-MCt has demonstrated that YB-1 gene was over-expressed by 2.86 fold in comparison to its expression in normal sample. This preliminary data is very important in the future experiment for expression of YB-I gene showed the potential as a biology marker for detection of brain tumor.
Description
Keywords
Cancer generally
Citation