Investigation of yb-1 gene expression by syber-green based real-time pcr in brain tumor.
| dc.contributor.author | Muhammad Redzwan, Sidik | |
| dc.date.accessioned | 2021-04-13T01:40:58Z | |
| dc.date.available | 2021-04-13T01:40:58Z | |
| dc.date.issued | 2008 | |
| dc.description.abstract | Cancer generally is a genetic disease that can cause alteration in specific gene in which a group of cells display uncontrolled growth, invasion and metastasis. YB-1 Gene Y -box binding protein (YB-1) is a member of the cold-shock domain (CSD) protein superfamily and involved in many cellular functions, including transcriptional regulation, translational regulation, DNA repair, drug resistance and stress responses to extracellular signals. YB-1 gene can be found in a cytoplasm and nuclear cell. The YB-1 gene comprises 8 exons spanning 19 kb of genomic DNA and is located on chromosome I p34. The aim of the study is to differential relatively quantitative the expression of YB-I gene in brain tumor sample (meningioma) and normal brain sample (Astrocyte SVG I2). SYBR-Green I based Real-Time PCR was performed and data analysis using formula 2-MCt has demonstrated that YB-1 gene was over-expressed by 2.86 fold in comparison to its expression in normal sample. This preliminary data is very important in the future experiment for expression of YB-I gene showed the potential as a biology marker for detection of brain tumor. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/12751 | |
| dc.language.iso | en | en_US |
| dc.publisher | Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia. | en_US |
| dc.subject | Cancer generally | en_US |
| dc.title | Investigation of yb-1 gene expression by syber-green based real-time pcr in brain tumor. | en_US |
| dc.type | Other | en_US |
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