The Genome And Recombinant Capsid Protein Of A Newly Isolated Escherichia Phage Yd-2008.S
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Date
2018-06
Authors
Sellvam, Dharmela
Journal Title
Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
The numbers of discovered phages are just a minute fraction of its population in the biosphere. However, the advancement of genome sequencing technology as NGS (next generation sequencing) provides opportunity to identify more complete phage genomes data that feasible to register them in ICTV (International Committee on Taxonomy of Viruses). A tailed phage species designated as Escherichia phage YD-2008.s was discovered and the complete genome was sequenced and deposited in NCBI (National Centre for Biotechnology Information) database with GenBank accession No. KM896878.1. Genomic sequence analyses revealed phage YD-2008.s genome that belongs to Siphoviridae phage family poses a linear dsDNA composed of 44,613 base pairs with 54.6% G+C content. Total number of sixty-two open reading frames (ORFs) were identified on phage YD-2008.s full genome, using bioinformatics annotation software: Rapid Annotation using Subsystem Technology (RAST). Among the ORFs, twenty-eight of them code for functional proteins. Thirty-two were classified as hypothetical proteins and there are two unidentified proteins. Even though majority of the coded putative proteins have high amino acids similarities to phages from the genus Hk578likevirus of the Siphoviridae family, yet phage YD-2008.s stands with its' own distinctiveness. As further verification of full genome sequencing results by NGS, capsid gene of phage YD-2008.s was expressed and analyzed. Thus, gene expression of the capsid gene further confirmed the authenticity of genome assembly, annotation and organization of phage YD-2008.s.
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Keywords
The genome and recombinant capsid protein , newly isolated escherichia phage YD-2008.s