The Genome And Recombinant Capsid Protein Of A Newly Isolated Escherichia Phage Yd-2008.S

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dc.contributor.authorSellvam, Dharmela
dc.date.accessioned2019-05-09T00:41:40Z
dc.date.available2019-05-09T00:41:40Z
dc.date.issued2018-06
dc.description.abstractThe numbers of discovered phages are just a minute fraction of its population in the biosphere. However, the advancement of genome sequencing technology as NGS (next generation sequencing) provides opportunity to identify more complete phage genomes data that feasible to register them in ICTV (International Committee on Taxonomy of Viruses). A tailed phage species designated as Escherichia phage YD-2008.s was discovered and the complete genome was sequenced and deposited in NCBI (National Centre for Biotechnology Information) database with GenBank accession No. KM896878.1. Genomic sequence analyses revealed phage YD-2008.s genome that belongs to Siphoviridae phage family poses a linear dsDNA composed of 44,613 base pairs with 54.6% G+C content. Total number of sixty-two open reading frames (ORFs) were identified on phage YD-2008.s full genome, using bioinformatics annotation software: Rapid Annotation using Subsystem Technology (RAST). Among the ORFs, twenty-eight of them code for functional proteins. Thirty-two were classified as hypothetical proteins and there are two unidentified proteins. Even though majority of the coded putative proteins have high amino acids similarities to phages from the genus Hk578likevirus of the Siphoviridae family, yet phage YD-2008.s stands with its' own distinctiveness. As further verification of full genome sequencing results by NGS, capsid gene of phage YD-2008.s was expressed and analyzed. Thus, gene expression of the capsid gene further confirmed the authenticity of genome assembly, annotation and organization of phage YD-2008.s.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8164
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectThe genome and recombinant capsid proteinen_US
dc.subjectnewly isolated escherichia phage YD-2008.sen_US
dc.titleThe Genome And Recombinant Capsid Protein Of A Newly Isolated Escherichia Phage Yd-2008.Sen_US
dc.typeThesisen_US
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