Clonal propagation of curcuma zedoaria rosc. and zingiber zerumbet smith {zingiberaceae)
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Date
2007-05
Authors
Christine
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Abstract
For establishment of aseptic rhizome buds of Curcuma zedoaria and
Zingiber zerumbe_t, the buds were surface sterilized with 100 mg/L mercury
Chloride (HgCI2) for five minutes followed by 20% (v/v) Clorox® with taw drops
of Tween 20 for 10 minutes in the first stage and 10% (v/v) Clorox® for 10
minutes in the second stage. This sterilization protocol enabled the production
of 87% aseptic and survived bud explants for C. zedoaria and 80% for Z.
zerumbet. The optimum shoot proliferation medium for both the species was
MS (Murashige and Skoog, 1962) medium supplemented with 0.5 mgll BA and
0.5 mg/L IBA. Shoot expiants of both species cultured in liqu!d proliferative
medium produced twice the number of multiple shoots than solid proliferative
medium. The suitable period of subculturing for these species was found to be
eight weeks. Continuous subculturing of these species caused a decrease in
the number of multiple shoots formed. For C. zedoaria, divided shoot explants
produced significantly higher numb_er of shoots than undivided or whole shoots.
But for Z. zerumbet the number of multiple shoots produced by divided shoots
was lower than the undivided or whole shoots. In vitro plantlets of both species
were successfully acclimatized to the soil with 100% survival rate. The divided
shoot explants of C. zedoaria produced 3.5 shoots per half shoots explant after
being immersed in the liquid proliferative medium for 15 minutes for two weeks
using temporary immersion system (TIS). For C. zedoaria. shake flask system
produced more number of multiple shoots than TIS. For Z. zerumbet there was
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no significant difference in the number of multiple shoots produced by TIS and
shake flask system. There was significant difference in the number of multiple
shoots of C. zedoaria and Z. zerumbet produced in proliferation medium (MS
medium plus 0.5 mg/L BA and 0.5 mg/L lBA) plus 30 g/L and 15 g/L sucrose
using TIS. However, there was no significant difference in the fresh weight and
dry weight of the multiple shoots of C. zedoaria and Z. zerumbet produced in
the proliferation medium plus 30 g/L and 15 g/L sucrose using T!S. In shake
flask system, there was also no significant difference in the number, fresh
weight and dry weight of the multiple shoots of both the species produced in
proliferation medium plus 30 g/L sucrose and 15 g/L sucrose.
Description
Keywords
Curcuma zedoaria rosc. , Zingiber zerumbet