Clonal propagation of curcuma zedoaria rosc. and zingiber zerumbet smith {zingiberaceae)
| dc.contributor.author | Christine | |
| dc.date.accessioned | 2015-09-03T07:16:08Z | |
| dc.date.available | 2015-09-03T07:16:08Z | |
| dc.date.issued | 2007-05 | |
| dc.description.abstract | For establishment of aseptic rhizome buds of Curcuma zedoaria and Zingiber zerumbe_t, the buds were surface sterilized with 100 mg/L mercury Chloride (HgCI2) for five minutes followed by 20% (v/v) Clorox® with taw drops of Tween 20 for 10 minutes in the first stage and 10% (v/v) Clorox® for 10 minutes in the second stage. This sterilization protocol enabled the production of 87% aseptic and survived bud explants for C. zedoaria and 80% for Z. zerumbet. The optimum shoot proliferation medium for both the species was MS (Murashige and Skoog, 1962) medium supplemented with 0.5 mgll BA and 0.5 mg/L IBA. Shoot expiants of both species cultured in liqu!d proliferative medium produced twice the number of multiple shoots than solid proliferative medium. The suitable period of subculturing for these species was found to be eight weeks. Continuous subculturing of these species caused a decrease in the number of multiple shoots formed. For C. zedoaria, divided shoot explants produced significantly higher numb_er of shoots than undivided or whole shoots. But for Z. zerumbet the number of multiple shoots produced by divided shoots was lower than the undivided or whole shoots. In vitro plantlets of both species were successfully acclimatized to the soil with 100% survival rate. The divided shoot explants of C. zedoaria produced 3.5 shoots per half shoots explant after being immersed in the liquid proliferative medium for 15 minutes for two weeks using temporary immersion system (TIS). For C. zedoaria. shake flask system produced more number of multiple shoots than TIS. For Z. zerumbet there was xviii no significant difference in the number of multiple shoots produced by TIS and shake flask system. There was significant difference in the number of multiple shoots of C. zedoaria and Z. zerumbet produced in proliferation medium (MS medium plus 0.5 mg/L BA and 0.5 mg/L lBA) plus 30 g/L and 15 g/L sucrose using TIS. However, there was no significant difference in the fresh weight and dry weight of the multiple shoots of C. zedoaria and Z. zerumbet produced in the proliferation medium plus 30 g/L and 15 g/L sucrose using T!S. In shake flask system, there was also no significant difference in the number, fresh weight and dry weight of the multiple shoots of both the species produced in proliferation medium plus 30 g/L sucrose and 15 g/L sucrose. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1151 | |
| dc.language.iso | en | en_US |
| dc.subject | Curcuma zedoaria rosc. | en_US |
| dc.subject | Zingiber zerumbet | en_US |
| dc.title | Clonal propagation of curcuma zedoaria rosc. and zingiber zerumbet smith {zingiberaceae) | en_US |
| dc.type | Thesis | en_US |
Files
License bundle
1 - 1 of 1
Loading...
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: