Phytochemical And Biological Investigation On Three Species Of The Genus Homalomena
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Date
2011-11
Authors
., Abdul Hamid
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Three species from the genus Homalomena, namely, H. pineodora, H. sagittifolia and
H. coerulescens were chosen for this particular research since they have long been used
in traditional medicine. The essential oils of the leaves and rhizomes of the three
species were isolated by hydrodistillation and analyzed by capillary GC and GC-MS,
utilizing two columns of different polarities. Isolation and purification of non-volatile
phytochemicals were carried out using various solvent systems and chromatographic
methods and their structures were determined by using spectroscopic methods such as
FT-IR, one and two dimensional NMR experiments: 1H-NMR, 13C-NMR, DEPT 45,
DEPT 90, DEPT 135, 1H-1H-COSY, 1H-1H-NOESY, 1H-13C-HMQC, 1H-13C-HMBC,
GC-MS, DP-MS and LR-MS. The essential oils, crude extracts and some of the isolated
compounds were assayed for biological activity (antibacterial, acetylcholinesterase
enzyme inhibitory activity and anti-inflammatory activity) to evaluate their reported
traditional uses.
The leaf and rhizome oils of H. pineodora contain high levels of non-terpenoid
compounds, particularly, (E)-tridec-2-en-4-one (47.5%) and 2-hydroxy-4-tridecanone
(29.5%), and these were isolated by using preparative GC and their structures were
established by means of spectral interpretation and confirmed by comparing their
spectral data with those of authentic standards. In addition, two constituents, tridec-1-
en-4-one and tridecane-2, 4-dione of H. pineodora were also identified by synthesis.
The essential oils of H. sagittifolia and H. coerulescens were dominated by
monoterpenoids. β-Pinene (16.5 % ) and linalol (34.5 %) were the major constituents
of the rhizome oil, while α-pinene (50.1 %) and β-pinene (24.1 % ) were predominant
in leaf oil of H. sagittifolia. In H. coerulescens, the main chemical component in the
rhizome oil was linalool (37.2 %), while in the leaf oil α- pinene (34.3%) and β- pinene
(24.9%) were predominant.
Description
Keywords
Phytochemical and biological investigation , three species of the genus Homalomena