Isolation, Genome Sequencing, Assembly, Annotation And Characterization Of Thermus Sp. Ccb_Us3_Uf1 From Ulu Slim, Perak, Malaysia
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Date
2011-02
Authors
Teh, Beng Soon
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Thermus sp. CCB_US3_UF1, a thermophilic bacterium has been successfully
isolated from a hot spring in Ulu Slim, Perak, Malaysia. The genome consists of
2,243,772 bp and a 19,716 bp plasmid. There are 2293 predicted coding sequences
(ORFs), 2 rRNA operons, 13 transposase genes, and 48 tRNA genes for all 20 amino
acids in the genome. The genome annotation predicts that the tricarboxylic acid
(TCA) cycle is complete. All the proteins/enzymes for carbohydrate metabolism
pathways are present. Thermus sp. CCB_US3_UF1 employs the non-oxidative
pentose phosphate pathway for the biosynthesis of ATP, histidine and coenzymes.
All biosynthetic genes for valine, leucine, and isoleucine are present, implying that
this bacterium can generate its own essential amino acids needed for growth. Several
enzymes of the Calvin cycle were identified, indicating that Thermus sp.
CCB_US3_UF1 may be able to fix CO2. However, due to the absence of the key
enzyme of this pathway, ribulose 1,5-biphosphate carboxylase/ oxygenase
(RUBISCO), alternative carbon-fixation pathways may be utilized. Regulation of
encoded genes DnaJ-DnaK-GrpE, GroEL-GroES, repressor HrcA, disulfide bond
chaperone of HSP33 family, chaperone HtpG, and small heat shock protein of
HSP20 family helps Thermus sp. CCB_US3_UF1 to adapt to a high temperature
environment. Proteases such as HslV, HslU, Clp, and Lon are found in Thermus sp.
Description
Keywords
Thermus Sp. , Ccb_Us3_Uf1